Can the proteomics dataset be used as is or do any additional filters need to be applied? How to check qc of the data.

Comments

6 comments

  • Comment author
    Ondrej Klempir DNAnexus Team

    Here is recently shared info about Olink data: https://community.dnanexus.com/s/question/0D582000000jnRACAY/new-data-release-on-ukbrap-olink-proteomics-data

     

    Data documentation is then available here: https://biobank.ndph.ox.ac.uk/showcase/label.cgi?id=1839, see the Resources tab

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  • Comment author
    Alexandra Lee DNAnexus Team

    This preprint details (in the supplementary material) the QC that was performed on the data: https://www.biorxiv.org/content/10.1101/2022.06.17.496443v1.full.pdf

     

    However, the existing QC and filtering already performed doesn't preclude any additional custom filtering/QC that you may want to perform that will probably depend on your research question

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  • Thank you very much for the prompt response. If I understand correctly, the metabolomics profiles are similar, excluding the quality control steps that are specific to the research question, is that right?

     

    Additionally, I am also seeking to obtain the raw proteomics values for a specific set of proteins. I have noticed that all the values are in NPX format. Do you happen to have any suggestions on how to retrieve the raw values from NPX?

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  • Comment author
    Alexandra Lee DNAnexus Team

    Personally I'm not very familiar with the metabolomics data.

     

    I believe that only NPX data will be released. However, there is only minimal pre-processing between the raw NGS counts and the NPX values, where the data was background corrected according to the preprint description - normalized to correct for background and then adjust to control for within and across batch effects.

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  • Comment author
    Rachael W The helpers that keep the community running smoothly. UKB Community team Data Analyst

    It is not possible to retrieve the raw values from the NPX values, and there is no intention to release the raw values in the forseeable future.

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  • Thank you so much for the update.

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